Prevention of Hospital Infection Exogenous infection. - презентация

1 Prevention of Hospital Infection Exogenous infection

2 Hospital Infection Surgical wards Operation Theaters Surgical Instruments

3 Surgical wards Should be well ventilated and cleaned The floor should be scrub washed once a week Preop. Pts should not be mixed with postop. Pts. Severely infected cases should be isolated Immuno suppressed pts also isolated

4 Operation Theaters Adjacent to ICU Height – 3M. Floor area 10 sqM. Anaesthetic room -10 Sqm. Minimise bacterial contamination The concept Site – away from the wards of zones : a) outer zone – reception area. b) clean zone – between the reception and OR. C) asceptic zone – OR. D) dirty zone – disposal area and space for cleaning of instruments 0

5 Air flow of OR Filtered air – directed vertically or horizontally Ideal – laminar flow Restrict entry of nonessential personnel & their movements Temperature – °C

6 Other Measures Wearing of disposable nonwoven fabric Masks Head & beard cover Preparation of the surgical team Preparation of the patient Preoperative hospitalisation minimised Skin infection treated Hair removal Skin preparation, Drapes

7 Disinfection The process that reduces the no. of viable microorganisms, but does not affect the spores

8 Disinfection Three levels of disinfection 1. Low: reduces the overall no. of vegetative microorganisms. Does not destroy TB bacilli or bacterial spores.Application – environmental surfaces 2. Intermediate : kills TB bacilli, most viruses,and some fungi but only some spores. Application – horizontal surfaces,floors 3. High: kills most forms of microbial life including TB bacilli but not some spores. Application – flexible endoscopes

9 Disinfection Heat disinfection 1) Pasteurization 2) Boiling 3) Low temperature steam disinfection

10 Chemical disinfection Chemical disinfectants are used to reduce the pathogenic microbes of inanimate objects, which are heavily contaminated. Alcohols, aldehydes, halogens, phenolics and quaternary ammonium compounds

11 Chemical disinfectants Alcohol – isopropyl alcohol & ethyl alcohol – kills most vegetative bacteria in less than 30 seconds, relatively inactive against spores and fungi. 70% alcohol is usually used. Aldehydes – glutaraldehyde & formaldehyde – good activity against spores, bacteria, virus and fungi. 2% glutaraldehyde rapidly inactivates HIV and hepatitis B after exposure for 10 minutes.

12 Chemical disinfection Diguanides – e.g. chlorhexidine – good activity against gm + ve organisms, moderate activity against gm- ve organisms, poor activity > TB bacilli, spores, fungi & viruses. Used as an antiseptic for skin & mucous memb. Halogens e.g. hypochlorites and chlorine – active against bacteria including spores,fungi, hepatitis B & HIV virus

13 Chemical disinfection Iodophores -1% available Iodine mixed with alcohol excellent skin preparation Phenolics – e.g phenol &chlorxylenol surface cleaning – Hexachlorophene – preparation of surgeon's hands Quaternary ammonium compounds – good detergent properties – savlon when mixed with chlorhexidine

14 Sterilization Complete removal of all microbes including spores Methods : Heat method – dry or moist Ionization radiation Ethylene oxide gas

15 Heat Methods Dry heat – Hot air ovens which have controlled cycles, such as 160°C for 1 hour – suitable for killing bacteria on materials which are not penetrable by steam e.g. glassware, powder such as talc, oils & petroleum jelly. Moist heat – Steam under pressure – simplest is domestic pressure High pressure vacuum autoclaves Low temp. steam(73°C) & formaldehyde – suitable for heat sensitive materials and equipment with plastic component

16 Ionising Radiation Gamma rays – lethal, noncharged, ultrashort, wavelength rays with great penetrating power from a radioactive isotope such asCobalt-60 e.g.Disposable syringes, sutures and rubber gloves The radiation dose 2.5 mrad – kills bacteria by ionising their DNA.

17 EthyleneOxide It should be used with suitable humidity at the correct temp. for sufficient time CO2 is mixed with ethylene oxide to reduce Highly toxic inflammable gas – kills all types of microbes including spores. the risk of explosion It diffuses well through items such as plastic materials, swabs and paper.