FUTURE PROJECTS Project 1 Preparing of small colloidal particles of noble metal with extremely small diameter Development of preparation technique of Au, - презентация

1 FUTURE PROJECTS Project 1 Preparing of small colloidal particles of noble metal with extremely small diameter Development of preparation technique of Au, Ag, Pt, Pd (maybe Cu, Ni) colloidal particles with the size less 10 nm using wet chemical synthesis with following reductors: N 2 H 4. H 2 O, N 2 H 4. BH 3, NaBH 4, HCHO, NaH 2 PO 2, (CH 3 ) 2 NH. BH 3, (C 2 H 5 ) 2 NH. BH 3, methol, pyrogallole a.a. (It is very important to protect from producing of boron compounds in the metals). Media : water, alcohol. Protective polymers: Polyvinyl alcohol, gelatin, polyethyleneglycole, polyvinylpyrollydone, a.a. Stabilizations: surfactants, pyridine, citrate ions, a. a. Investigation: type reductor, pH of solution, potential of solutions and particles, size of colloidal particles, spectrum of absorption, optimization of preparation for creation of minimal size (temperature, solvent, polymer or protein protective media) stability of colloidal systems during a rather long time. Creation of hybrid particles like Au/Ag, Pd/Ni with the help of physical development. Project 2 Sonochemical preparing of small colloidal particles of metals and oxides Development of preparation technique of colloidal particles with the size less 300 nm using sonochemical synthesis for metals and their oxides. Types of metals: Pt, Ni, Fe, Co, Cu, W, a. a. Media: hydrocarbons, alcohols, water. Atmosphere: inert gases (Ar, N 2 ), oxygen O 2. In the inert atmosphere we can create metal particles, in oxygenic one the particles of their oxides. Substances for sonochemical synthesis: carbonyls, diketonates and other easily evaporable compounds of metals. Stabilizations: polyethyleneimine, surfactants, pyridine, citrate ions, a. a. Investigations: influence on the particles dispersity of type of metals, media, stabilizations, power and frequency of ultrasonic oscillator, temperature and duration of process. Investigation of stability in organic (oxygen-less) solvents, water, on air. Creation hybrid particles by consecutive synthesis.

2 FUTURE PROJECTS Project 3 Using of small colloidal particles as markers of biologically active places onto the cells surface Producing of colloidal particles of noble metals of different sizes, labeled their with the biologically active substances, investigation of topography of active places onto the cells surface with the help of such colloidal particles with high electron density as markers and by using of unique carbon replica technique. Simultaneously investigation of several types of biological active places with the help of several sizes or several types of colloidal particles. Types of metals: Au, Pt, Ag. Size of particles: 10 nm, 5 nm and less. Biological substances: antibodies, receptors, different types of cells proteins, a. a. Investigation of processes: phagocytosis, erythrocytes-macrophages or lymphocytes-macrophages rosettes, antigen-antibody interactions, detection of pathogenic bacterium a. a. Investigation of 3D surfaces of cells. Additional task: Creation of conductive nano-fibers or nano-tubes from self-assembled protein labeled gold colloidal particles. Project 4 Langmuire films of amphiphilic polymers as protective layers by forming and transferring of active films of biological substances Development technique preserving of biological molecules against the destruction effect by transforming through the water surface by immobilization onto the substrate. We propose the Langmuire technique and protective layers of amphiphilic polymers. Types of polymers: polyethyleneimine, polyvinyl-pyrrolidone, polyacids (like polyacrilic acid) modified with hydrocarbons chains. Types of biological substances: enzymes, antibodies, receptors a. a. Investigation of processes: forming of Langmuire films, isotherms of adsorption, temperature and duration of process, process of interaction between monomolecular film and proteins molecules. Investigation of protein-polymer interaction process in volume and onto the water sub-phase surface.

3 FUTURE PROJECTS Project 5 Creation of small colloidal particles for new generation of biosensors based on BARC format Synthesis colloidal particles covered by polymeric layers for protect from media, for easy labeled by proteins, for creation of stabile suspension in the physiology liquids for Beads ARray Counter format. Particles from: magnetite, gold or silver. Detection by: gigant magnetic resistance effect sensors, transmitted electron microscope. Investigations: process of polymerization onto the magnetic particles surface and different types of inclusion of particles into the polymer core, stability of suspension, charge of particles, adsorption of proteins onto the particles surface, stability of biological activity. Increasing of stability with the help of different types of polymers like branched and charged polycations or polyanions. Project 6 Langmuir 2D crystal films of biological substances Creation of 2D films from proteins for electron microscopy investigation of structure and chains conformation. Investigation: influence the various types of drug or perfume substances onto the protein 2D structure. Using technology: Langmuir films creation, adhesion to lipid film, creation of amphiphilized proteins. We have not enough of experience in this area –the creation only 2D films of ferritin.

4 FUTURE PROJECTS Project 7 Investigation of new phase generation and real structure of point-defects in pure crystals or in the crystals with special entered impurities Purpose of project: investigation of new phase (natural and specially entered) generation process in the crystals using of vacuum decoration methods of point defects with colloidal particles on electron microscope level. Crystals: ultra-fine-pure KCl, NaCl, ZnO, LiF, SiO 2 or crystals with special entered components. Impurities: natural OH - ; special entered: Cation 2+, Anion 2- ; F-centers by γ-irradiation and addition coloring in the vapors of metals. Types of colloidal particles: Au, Ag, AgCl, AgBr, PbS, Ga. Project 8 Investigation of formation of new silver phase during photolysis and physical and chemical developments in ultra-small-grained silver halide micro crystals and during additional treatment Purpose of project: investigation of process of formation of new colloidal silver phase and oligoatomic silver clusters in ultra-fine-grained AgBr crystals of transparent Lippmann photoemulsions by photolysis, chemical and physical development. Crystals: AgBr with size of 0 to 200 nm. Colloidal particles of Ag with size of 3 to 30 nm. Clusters of Ag of 1 to 10 atoms.