New methods for the production of monoclonal antibodies and their fragments. Made by : Dossova Alma Specialty: Biotechnology Group:

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New methods for the production of monoclonal antibodies and their fragments. Made by : Dossova Alma Specialty: Biotechnology Group:

Plan What is a monoclonal antibody? The standard procedure for the production of monoclonal antibodies (Hybridoma technology) Obtaining immune B-lymphocytes in humans. The production of infinitely dividing cells- producers of human antibodies (immortalization). The use of genetic engineering methods for the production and production of human antibodies. Use of phage libraries (displays) for the production of MAB. Use of transgenic mice to produce human mAbs.

What is a monoclonal antibody? Monoclonal antibodies - are antibodies produced by immune cells belonging to a single cellular clone, that is, against one particular determinant of the antigen.

Hybridoma technology

Obtaining immune B-lymphocytes in humans

The production of infinitely dividing cells-producers of human antibodies (immortalization) Immortalization - is the obtaining of a stable, capable of unlimited reproduction (an "immortal") cell line from cells with a limited lifetime in culture; Immortalization - is one of the stages of oncogenesis.

The use of genetic engineering methods for the production and production of human antibodies.

Use of phage libraries (displays) for the production of MAB.

Use of transgenic mice to produce human mAbs.

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